The tender-clipped sequences that overhang the breakpoint are collapsed into a consensus sequence utilizing a numerous sequence alignment (MSA) furnished while in the seqAn library [27]. Wham applies a few filters to your consensus sequences. Breakpoints are not claimed in conditions exactly where consensus sequences are shorter than 10 bp or consi

Wham clusters the many prospect breakpoints and rounds their positions to the nearest tenth foundation pair. The place with the highest read through support is described. If your gentle-clipped consensus sequence might be aligned to your putative breakpoint location using the Smith-Waterman algorithm, the breakpoint is further more refined to The p

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